The absorbance of a 1 mg/ml sample of lysozyme was measured in a cuvette of thickness...

The absorbance of a 1 mg/ml sample of lysozyme was measured in a cuvette of thickness 1.00 cm and was found to be 3.19 at 240 nm and 2.58 at 280 nm. The molar absorption coefficients of tryptophan and tyrosine at 240 nm are 2.00 x 103 dm³ mol^-1 cm^-1 and 1.12 x 104 dm³ mol^-1 cm^-1, respectively, and at 280 nm they are 5.40 x 103 dm3 mol^-1 cm^-1 and 1.50 x 103 dm³ mol^-1 cm-1, respectively. What are the molar concentrations of the two amino acids in the 1 mg/ml protein sample? If the molar mass of the protein is 14,400 g/mol, how many tryptophans and tyrosines are present in the sequence of this protein? (looking for the molar ratio, moles aa/mol protein) What is the molar absorption coefficient of the lysozyme protein at 280 nm? What would be the % Transmittance of the same protein sample if measurements were made in a 0.1 cm cuvette at 280 nm?

Expert Solution

queen_honey_blossom answered 8 months ago

The absorbance of a cationic iron(II) sample solution was measured in a spectrophotometer, but the instrument...

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For a sample containing both protein and nucleic acid, the absorbance measured at a particular wavelength...

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Researchers measured the cholesterol levels in mg per 100 mL for 50 participants. The participants were...

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The concentration of an undiluted sample is 26.4 mg/ml. To calculate that answer, the concentration of...

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Why is it important that all absorbance readings for the calibration curve be measured at the...

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A 125.0 mg sample of methylamine (CH3NH2 MW = 31.06) was dissolved in 25.00 mL of...

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Being provided are: • 50mM Tris Buffer • 50mg/ml Lysozyme • 20mg/ml DNase • 0.5M NaCl...

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The following questions refer to Quantum Dot samples (CdSe). We measured absorbance with a spectrophotometer. 1....

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Absorbance at 540 nm of Bromocresol Purple Dilution Series test tube concentration of sample (ug/mL) A540...

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