The absorbance of a 1 mg/ml sample of lysozyme was measured in a cuvette of thickness...

The absorbance of a 1 mg/ml sample of lysozyme was measured in a cuvette of thickness 1.00 cm and was found to be 3.19 at 240 nm and 2.58 at 280 nm. The molar absorption coefficients of tryptophan and tyrosine at 240 nm are 2.00 x 103 dm³ mol^-1 cm^-1 and 1.12 x 104 dm³ mol^-1 cm^-1, respectively, and at 280 nm they are 5.40 x 103 dm3 mol^-1 cm^-1 and 1.50 x 103 dm³ mol^-1 cm-1, respectively. What are the molar concentrations of the two amino acids in the 1 mg/ml protein sample? If the molar mass of the protein is 14,400 g/mol, how many tryptophans and tyrosines are present in the sequence of this protein? (looking for the molar ratio, moles aa/mol protein) What is the molar absorption coefficient of the lysozyme protein at 280 nm? What would be the % Transmittance of the same protein sample if measurements were made in a 0.1 cm cuvette at 280 nm?

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queen_honey_blossom answered 1 month ago

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