Question

"For a minimus of 2 pages, discuss the 1. Structure and 2. Post-translational modifications of the following protein:

Gene Name: CDK2

Uniport: P24941

Don't forget to include references."

Please help!

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Answer 1

GENE NAME: CDK2 UNIPORT P24941: CYCLIN- DEPENDENT KINASE2: THE MONOMERIC CDK2 CONSISTS OF 298 AMINO ACIDS, AND FOLDS INTO A TYPICAL BILOBIAL STRUCTURE, WITH A SMALLER N- TERMINAL LOBE ( RESIDUES 1- 185 ) LOBE PREDOMINANTLY COMPOSED OF A APLHA - HELIX (66). ACTUALLY, THE TWO TERMINAL DOMAINS ARE CONNECTED THROUGH A SINGLE PEPTIDE STRAND ( RESIDUES 81-83 ) WHICH ACTS ASA HINGE LINKED TO ENSURE THAT THE TWO LOBES CAN ROTATE WITH RESPECT TO EACH OTHER WITHOUT DISRUPTION OF SECONDARY STRUCTURE OF THIS KINASE (67). THERE ARE TWO IMPORTANT SEGMENTS IN THE LARGE C -TERMINAL DOMAIN, INCLUDING THE CATALYTIC RESIDUES THAT ARE IN CHARGE OF PHOSPHORYLATION PROMOTION, AS WELL AS THE ACTIVATION SEGMENT. AMONG THEM, THIS ACTIVATION SEGMENT SPANS RESIDUES BETWEEN THE CONSERVED GLYCINE - RICH LOOP. .THE T LOOP WHICH IS LOCATED IN THE ENTRANCE OF THE CATALYTIC CLEFT, HOLDS THE BACK THE POLYPEPTIDE SUBSTRATES AND, IN THIS WAY PREVENTS THEM FROM ENTERING INTO THE CLEFT INTO INTERACTWITH ATP.

POST TRANSLATIONAL MODIFICATIONS OF CDK2 GENE.

IT IS PHOSPHORYLATED AT Thr -160 BY CDK7 IN A CAK COMPLEX . PHOSPHORYLATION AT Thr-160 PROMOTES KINASE ACTIVITY,( PUB MED: 1396589) WHEREAS PHOSPHORYLATION AT Tyr- 15 BY WEE1 REDUCES SLIGHTLY KINASE ACTIVITY. PHOSPHORYLATED ON Thr- 14 AND Tyr -15 DURING S AND G2 PHASES BEFORE BEING DEPHOSPHORYLATED BY CDC25A .