Whats the difference between Random shotgun sequencing and target sequencing of marker genes such as 16...

Whats the difference between Random shotgun sequencing and target sequencing of marker genes such as 16 rRNA. Be concise.

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16S rRNA utilizes PCR to target amplify portions of the hypervariable regions (V1-V9) of the bacterial 16S rRNA gene.Amplicons from separate samples are then given molecular barcodes,pooled together and sequenced.

Unlike 16S sequencing,which only target 16S rRNA genes,shotgun sequencing sequences all given genomic DNA from a sample.It includes random fragmentation and adapter ligation.

Overall shotgun has greater taxonomy resolution, functional profiling and cross-domain coverage.In all other aspects including price and sample origin compatibility,16S sequencing has the advantage.

gladiator answered 7 months ago

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Whats the difference between Random shotgun sequencing and target sequencing of marker genes such as 16...

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