Which of the following statements correctly contrasts DNA pol III with a translesion DNA polymerase, such...

Which of the following statements correctly contrasts DNA pol III with a translesion DNA polymerase, such as DNA pol IV or V? explain the answer please for each statement.

1. Translesion DNA polymerase has low fidelity, while DNA pol III has high fidelity.

2. DNA pol III is highly processive, but translesion polymerases are not.

3. DNA pol III requires a template strand of DNA, unlike translesion polymerases.

4. DNA pol III polymerizes DNA in a 5' to 3' direction, while translesion polymerases build DNA in the opposite direction.

5. DNA pol III requires an exposed 3' end of a primer strand to synthesize DNA, while translesion polymerases can initiate synthesis from scratch.

Expert Solution

Answer is attached with explanation

Correct option is 1 and 2

gladiator answered 3 years ago

Assuming DNA polymerization by each polymerase in the DNA Pol III holoenzyme proceeds at a rate...

Assuming DNA polymerization by each polymerase in the DNA Pol III holoenzyme proceeds at a rate of 1000 nucleotides per second, how long would anormal E.coli cell take to replicate the entire E .coli chromosome? Under optimal condition, E.coli cells divide every 20minutes. How many replication forks will be present in each E.coli to sustain such a rate of division?

E.Coli DNa Pol III consists of a 5'3' polymerase activity and a 3'5' proofreading activity. lets...

E.Coli DNa Pol III consists of a 5'3' polymerase activity and a 3'5' proofreading activity. lets assume that a novel mutation results in a defective DNA Pol III, which can normally Polymerize nucleotides But can't proofread accurately. This error prone polymerase may result in one mistake per 100 base pairs. In order to prevent extensive damage to the genome, the micro must recruit a DNA repair mechanism. The microbe should be able to identify the newly synthesized strand of DNA...

Explain what would happen during DNA replication if both DNA Pol I and DNA Pol III...

Explain what would happen during DNA replication if both DNA Pol I and DNA Pol III had high processivity. in writing no diagrams or pictures

The β (beta) subunit of E. coli DNA polymerase III possesses which of the following enzymatic...

The β (beta) subunit of E. coli DNA polymerase III possesses which of the following enzymatic activities? 1. 3’- 5’ polymerase 2. 3’- 5’ exonuclease 3. 5’ - 3’ exonuclease 4. 5’ - 3’ polymerase 5. the beta subunit is not an enzyme

1. Why does E. coli need both DNA polymerase III and DNA polymerase I? a. The...

1. Why does E. coli need both DNA polymerase III and DNA polymerase I? a. The DNA replication is bidirectional; one polymerase is used for each direction. b. Each polymerase is specific for only one strand of DNA. DNA polymerase III acts only on the leading strand, and DNA polymerase I acts only on the lagging strand. c. Only DNA polymerase I has proofreading ability. d. DNA polymerase III lacks the 5' → 3' exonuclease activity needed to remove RNA...

Which of the following is not a property of DNA polymerase?Which of the following is a...

Which of the following is not a property of DNA polymerase?Which of the following is a property of DNA polymerase?It requires a primer to work.Its exonuclease activity is involved in proofreading.It adds dNTPs only in the 3' to 5' direction.It is associated with a sliding clamp both on the leading and lagging strands.

Scientists decided to study, in vitro, the activity of the eukaryotic DNA polymerase Pol e. 1)...

Scientists decided to study, in vitro, the activity of the eukaryotic DNA polymerase Pol e. 1) In a first experiment, they apply a solution of Pol e on top of a chromatography column containing heparin. They collect the solution that elutes from the bottom of the column (flow-through). Their analysis reveals that the flow-through does not contain any protein. What is the property of heparin demonstrated by this experience? 2) They designed a second experiment including 4 steps. Step 1:...

Eukaryotic cells produce three distinct RNA polymerases: pol I, pol II, and pol III. Which of...

Eukaryotic cells produce three distinct RNA polymerases: pol I, pol II, and pol III. Which of the following statements is NOT true about eukaryotic RNA polymerases? A) Pol II, with twelve subunits on its own, is capable of initiating transcription. B) Pol III is responsible for the transcription of all tRNAs. C) Pol I carries out the transcription of the 45S pre-rRNA transcript. D) Pol III is responsible for the transcription of ribosomal 5S rRNA.

What would happen if we use Polymerase I instead of Polymerase III for DNA replication in...

What would happen if we use Polymerase I instead of Polymerase III for DNA replication in a PCR? If we used an enzyme with low fidelity, how would the DNA product compare to the original template?

DNA polymerase III builds new DNA strands in the 5' to 3' direction...always adding the new...

DNA polymerase III builds new DNA strands in the 5' to 3' direction...always adding the new nucleotide to the 3' end of the existing strand. As it adds new nucleotides, it proofreads its work. If a mistake is detected, DNA polymerase will act as an exonuclease and excise the incorrect nucleotide. A. What supplies the energy needed to add the new nucleotide to the existing chain? B. Please explain why evolution has favored 5’ to 3’ polymerases, as opposed to...

Question