Question

Match the following descriptions to the most appropriate method of genome engineering presented within the list. Use each answer once. List: MuGENT, Transformation, CRISPR, Transposon mutagenesis, and MAGE.

a. You are attempting to see the effect of substituting similar amino acids across the genome (called conservative replacement). Thus, by using _____ you try and replace all glycine codons with alanines.

b. You are looking to create a heterogenous mixture of knockout mutants from a pool of 6 genes.

c. A broadly-applicable technique that can make efficient gene edits based on guide RNA.

d. You are looking to randomly disrupt genes within the genome to assess their function.

e. Using a PCR fragment with homology to the genome, you must get this DNA into your target cells somehow...

Answer 1

a) Transposon Mutagenesis (This method we use through creation of mutation or changes on the DNA sequences with the help of jumping gene or transposons another method of mutagenesis is through PCR for certain changes in nucleotide cause in the amino acid).

b) MAGE: This is a Multiplex method for automated genome engineering, another tool being used in molecular biology for construction of several changes or mutation a time (at different loci).

c) CRISPER (DNA sequences found in bacteria due to prior infection of bacteriophage, being used as an efficient tool in gene editing)

d) MuGENT: Multiplex editing of genome can be carried out by this technique particularly for disruption of random genes.

e) Transformation: Commonly used method in cloning (bacterial) which PCR use for the desired construct and later on transfer this construct to the target cell with the process known as transformation.