Of the following four 15-bp double-stranded DNA sequences, which will have a higher melting temperature? (Note:...

Of the following four 15-bp double-stranded DNA sequences, which will have a higher melting temperature? (Note: only one strand is shown here) Choose the single best answer
- CCCGCATCGCCATCG
- CATCCTAGCGACTAT
- CTATACGACATAGCC
- AAATGCATACATCTT

Solutions

Expert Solution

Melting point of DNA :-
• This is the temperature, at where double stranded DNA turns into single stranded DNA by breaking all hydrogen bonds between double strands.
• The DNA which has high number of hydrogen bonds has higher melting point.

Number of hydrogen bonds :-
• In double stranded DNA = Three hydrogen bonds present between guanine and cytosine, two hydrogen bonds present between adenine and thymine.
• So, now the DNA strand which has more numbers of cytosine-guanine base pairs, has more numbers of hydrogen bonds and has higher melting point.
• Here in question only single strand is given so, which single strand has more number of guanine and cytosine that has more guanine-cytosine base pairs.

Melting point of DNA ∝ Numbers of hydrogen bonds ∝ Numbers of guanine cytosine base pairs.

So, guanine cytosine base pairs in given DNA strands -
Strand 1 = 11 Guanine and cytosine bases.
Strand 2 = 7
Strand 3 = 7
Strand 4 = 4

Hence, Strand 1 has highest melting point.
Sequence of melting point :-
Strand 1> Strand 2= Strand 3> Starnd 4

gladiator answered 3 months ago

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