Question

7) Your partner is ready to use a gene drive to eradicate Zika. Your partner is proposing a flowchart for his experiment but she jumbled up his lab and field protocols. Place the following, applicable statements in order: (Example: d x f …. Etc..) Answer: ______ _______ ______ _____ ______ ______ ______ ______ _____ _____

A) Identify freshly emerging recombinant mosquitos that glow red under the fluorescent microscope.

B) After a few months do DNA tests on wild life and mosquitos on the island and find only very few (if any) Dengue-positive specimens.

C) Sit back in your beach chair, smile, and think of the fame that will be yours for eradicating Zika. What could possibly go wrong?

D) In the Lab, show that recombinant mosquito offspring can feed on mice carrying the Zika virus but afterwards do not carry the Zika virus themselves.

E) Show that the mosquito’s offspring also glow red and now contain the original gRNA, Cas9, and a monobody to the Zika pathogen.

F) On an uninhabited, tropical offshore island infested with Zika virus release hundreds of recombinant, red-eyed mosquitos.

G) Generate an expression construct with the matching gRNA, Cas9, RFP and a monobody to the Zika virus.

H) Rear the recombinant mosquitos on laboratory mice, let them grow and mate with wild-type mosquitoes.

I) Search the database to identify a Zika virus target sequence with a juxtaposed PAM

K) Micro-inject the construct into developing mosquito larva.

Answer 1

Answer:

Your partner is ready to use a gene drive to eradicate Zika. Your partner is proposing a flowchart for his experiment but she jumbled up his lab and field protocols. Place the following, applicable statements in order:

Below is the correct squence:

A) Search the database to identify a Zika virus target sequence with a juxtaposed PAM

B) Generate an expression construct with the matching gRNA, Cas9, RFP and a monobody to the Zika virus.

C) Micro-inject the construct into developing mosquito larva.

D) Identify freshly emerging recombinant mosquitos that glow red under the fluorescent microscope.

E) Rear the recombinant mosquitos on laboratory mice, let them grow and mate with wild-type mosquitoes.

F) Show that the mosquito’s offspring also glow red and now contain the original gRNA, Cas9, and a monobody to the Zika pathogen.

G) In the Lab, show that recombinant mosquito offspring can feed on mice carrying the Zika virus but afterwards do not carry the Zika virus themselves.

H) On an uninhabited, tropical offshore island infested with Zika virus release hundreds of recombinant, red-eyed mosquitos.

I) After a few months do DNA tests on wild life and mosquitos on the island and find only very few (if any) Dengue-positive specimens.

J) Sit back in your beach chair, smile, and think of the fame that will be yours for eradicating Zika. What could possibly go wrong?