What is the net charge of the polypeptide NYSCAS at ph 1,ph7,ph 13

Expert Solution

Ans. Calculation of Charge on peptide:

Note: Only the N-ter, C-ter, acidic and basic residues are used for calculating charge. [NYSCAS]

Method 1: Use Henderson-Hasselbalch equation

Net Charge (exact value) at pH 1.0 = 0.931

Net Charge (exact value) at pH 7.0 = - 0.018

Net Charge (exact value) at pH 7.0 = - 1.000

#I. The residues with acidic side chain and C-terminal are written in RED/BOLD. Their corresponding charge is written in columns of “Acidic residues”. Ignore all other charge/values in “black ink”. The sum of charge in “RED ink” is the total –ve charge on the peptide at specified pH.

#II. The residues with basic side chain and N-terminal are written in Blue/BOLD. Their corresponding charge is written in columns of “Basic residues”. Ignore all other charge/values in “black ink”. The sum of charge in “Blue ink” is the total +ve charge on the peptide at specified pH.

#III. The charge on each residue/ group is calculated using Henderson-Hasselbalch equation as shown in respective column.

#IV. Only residues with acidic and basic side chain are shown in excel for calculation.

queen_honey_blossom answered 2 years ago

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