Question (2) Which of the following histology stains is/are used to highlight glycosaminoglycans in tissue sections...

Question (2) Which of the following histology stains is/are used to highlight glycosaminoglycans in tissue sections

Alcian Blue
Millers elastin
Massons trichrome
Haematoxylin
Trypan blue

Expert Solution

Answer- A. Alcian Blue

Explanation- Millers elastin is a stain that used to stain elastic tissue. After staining, elastic tissue will take purple coloration under microscope.

Hence, option B is incorrect.

Whereas Massons trichome is generally used for staining collagen like connective tissue. Collagen tissue take its characteristic blue coloration.

Hence, option C is incorrect.

Haematoxylin is a stain that is used to stain nucleus of a cell. After completion of staining with haemtoxylin, nucleus become blue colored when seen under microscope.

Hence, option D is incorrect.

To determine the cell viabilty, scientists often used trypan blue stain. Cells which are dead they are blue in colour, whereas viable cell are not being stained by trypan blue.

Hence, option E is incorrect.

Glycosaminoglycans are basically mucopolysaccharides having disccharides as monomer. This stain is having high water solubility. Alcian blue helps to stain glyccosaminoglycans by binding with negatively charged molecule of glycosaminoglycans.

Hence, option A is correct

gladiator answered 1 year ago

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