How much of each of the solutions is needed to make the buffer? Final volume is...

How much of each of the solutions is needed to make the buffer? Final volume is 10 mL.

stock solutions

1 M HEPES (pH 7.9)

5 M NaCl

1 M BME

100 mM EDTA

750 mM MgCl2

50% glycerol

C) nuclear extraction buffer

20 mM HEPES

1 M NaCl

1 mM BME

0.5 mM EDTA

1.5 mM MgCl2

25% glycerol

Solutions

Expert Solution

queen_honey_blossom answered 9 months ago

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