An anion-exchange resin can be used instead of the
cation-exchange resin in this experiment to separate the three
amino acids in the known mixture. What changes to experimental
protocol are required to achieve complete resolution? Support your
response with an explanation. (HINT: consider pH).
You have been provided a mixture containing four amino acids
(Glu, His, Pro and Lys) to be separated by ion-exchange
chromatography using a Dowex 50 cation-exchange, prepared in a
loading buffer at pH 2.0. The following...