Question

Set IHMR A	Relative Concentration Methyl Red	Absorbance (a.u.) at 425 nm
A0	1.00	0.094
A1	0.75	0.058
A2	0.50	0.041
A3	0.25	0.030

Set IIHMR A	Relative Concentration Methyl Red	Absorbance (a.u.) at 520 nm
A0	1.00	0.895
A1	0.75	0.806
A2	0.50	0.597
A3	0.25	0.224

Set IMR- B	Relative Concentration Methyl Red	Absorbance (a.u.) at 425 nm
B0	1.00	0.480
B1	0.75	0.302
B2	0.50	0.243
B3	0.25	0.052

Set IIMR- B	Relative Concentration Methyl Red	Absorbance (a.u.) at 520 nm
B0	1.00	0.048
B1	0.75	0.033
B2	0.50	0.023
B3	0.25	0.017

Based on the data above, calculate the slope of the line of the absorbance vs relative concentration for each for the four data sets (Part III in your lab manual).

Let:

αHMR
IA equal the slope for solutions A₀-A₃ at 425 mm

αMR^-
IB equal the slope for solutions B₀-B₃ at 425 mm

αHMR
IIA equal the slope for solutions A₀-A₃ at 520 mm

αMR^-
IIB equal the slope for solutions B₀-B₃ at 520 mm

These α's are the respective Beer's Law constants for HMR and MR^- at the 2 wavelengths. Enter below the α's that you determined from your Excel graphs

Answer 1