Question

In: Other

Centrifuge Parts (match the part in Column A with the one correct function in Column B)...

Centrifuge Parts (match the part in Column A with the one correct function in Column B)

Column A

1. Rheostat ______

2. Bucket _______

3. Trunnion ring _____

4. Brake _______

Column B

A. sets spinning time

B. speed control

C. tube holder

D. holds bucket

E. allow for faster slow down time ?

Types of Centrifuges (match the Centrifuge name in Column A with the one correct function in Column B)

Column A

5. Cytospine

6. Horizontal head

7. Microheamatocrit

Column B

A. greater speed than angle head

B. retains good cellular detail

C. spins capillary tubes in hematology

D. better separation of layers

8. An advantage of a horizontal head centrifuge is (may be more than 1 answer)

a) gives best separation of layers

b) spins faster than angle head due to less wind resistance

c) holds more tubes

9. 8 tubes of whole blood were placed in a centrifuge. The speed was set for 2000 rpm for 10 minutes. The imbalance light came on as soon as the instrument started spinning. Choose from the list below all possible causes

a) rubber cushion missing from 1 or more buckets

b) tubes not placed opposite each other in buckets

c) blood volumes for opposite tubes not equal

d) tube sizes not all the same

10. If a method required spinning at an exact gravitational force, you must know the Relative Centrifugal Force for that centrifuge for a specific speed. The 2 things that will influence the RCF are: a) speed of rotation (RPM) b) size of the centrifuge (distance of tubes from motor shaft) c) nature of the samples to be spun RCF = 1.12R(RPM/1000)2, R= radius

11.State the steps to be followed if a tube of serum breaks in a centrifuge.

Solutions

Expert Solution

Centrifuge Parts (match the part in Column A with the one correct function in Column B)

Column A                       Column B

1. Rheostat                  Speed control

2. Bucket                     Tube holder

3. Trunnion ring          Holds bucket

4. Brake                    Allow for faster slow down time

Types of Centrifuges (match the Centrifuge name in Column A with the one correct function in Column B)

Column A                              Column B

5. Cytospine                         B. retains good cellular detail

6. Horizontal head                D. better separation of layers

7. Microheamatocrit             C. spins capillary tubes in hematology

8. An advantage of a horizontal head centrifuge is (may be more than 1 answer)

a) gives best separation of layers

c) holds more tubes

9. 8 tubes of whole blood were placed in a centrifuge. The speed was set for 2000 rpm for 10 minutes. The imbalance light came on as soon as the instrument started spinning. The list below is of all possible causes

b) tubes not placed opposite each other in buckets.

d) tube sizes not all the same.


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