i) Describe the components of a fusion protein. ii) Explain how fusion proteins are purified...

i) Describe the components of a fusion protein.

ii) Explain how fusion proteins are purified by affinity chromatography.

Expert Solution

gladiator answered 2 years ago

After running your purified DHFR fusion protein+Laemmli 40uL sample, along with a protein ladder (Kaleidoscope standard...

After running your purified DHFR fusion protein+Laemmli 40uL sample, along with a protein ladder (Kaleidoscope standard you used in lab), you transfer protein on the gel to a nitrocellulose filter for Western Blot analysis. *THE FUSION PROTEIN WAS IDENTIFIED TO BE HIS a) Which of the following primary antibodies could you use to detect your fusion protein based on the fusion protein identified and process described above: Anti-Myc, Anti-FLAG, Anti-GST, Anti-His, Anti-DHFR, Anti-Tubulin? List all that apply: b) List how...

Describe (i) how the process of molecular evolution generates protein families, and (ii) how the amino...

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briefly describe how the GST tag could be utilized to purify the fusion protein from a cell...

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