I am doing the lab of chromatography of spinach. Can you briefly explain what "sources of...

I am doing the lab of chromatography of spinach. Can you briefly explain what "sources of error" in this experiment? Limitation of technique and improvment? There is no any information is given. Just need some explains

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Expert Solution

Aim:

The purpose of the experiment is to determine the specific types of pigments found in a spinach leaf by using paper chromatography and two solvents: water soluble solvent and lipid soluble solvent.

Limitations:

1. Drawing on chromatography paper with pen. • Remind students that ink is a pigment just like chlorophyll. If they use a pen, their line will slowly move up the paper

2. Not transferring enough pigment onto the chromatography paper. • A generous amount of pigment is required in order to achieve clear separation.

3. Touching the leaf pigment to the alcohol at the bottom of the jar. • If the pigment touches the alcohol, it will immediately dissolve into the liquid at the bottom of the jar and will not move up the chromatography paper.

4. Not leaving the chromatogram in the alcohol long enough to allow the pigments to separate. • The chromatogram should be left in the jar until the solvent front (alcohol) reaches the top of the chromatography paper. This will take approximately 30 min.

Hope this helps!

queen_honey_blossom answered 1 year ago

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