Using a fluorescence microscope, you observed how the
localization of GFP-Ras changes in response to the inhibitor.
GFP is a reporter protein, what does this mean?
You used stains to visualize the nucleus and golgi bodies and
the fluorescence microscope to determine whether the GFP-Ras
protein was present in the golgi or nucleus. How do these stains
work?
Hoechst 34580
BODIPY TR Ceramide
a) Describe, using a schematic/diagram of your own creation, how
an electron microscope changes from low magnification to high
magnification.
b) Why is it preferable to image a conductive surface in an
SEM?
c) Is the conductivity of the sample as critical in a TEM as it
is in an SEM? Why or why not?
Since enzymes are essential in cellular function – it’s
important to regulate their activity. There are several ways a cell
can accomplish such regulation. How do cells regulate enzyme
activity? (required minimum length 150 words)
1. Describe the compound light microscope, what are the
function of the stage clips. Specify what type of objects or
organisms you would look at using each of these microscopes
respectively.
2. Identify two structures that are unique to the plant
cells and provide each of their functions. Describe what happens to
a plant cell when it is placed in a hypotonic solution and be sure
to state the name of this process.
3. Name the four factors that affect...
Using a well labeled diagram and words that refer to and
explain that diagram to describe the four steps necessary for a
monopolist to select its price and output to maximize profits in
the short run?
Describe the general function of teeth. Diagram the general
structure of a tooth. Describe the different kinds of specialized
teeth found in mammals. For each type of tooth provide the type of
diet it is associated with.