In: Chemistry
A 0.2257g sample of standard steel contains 0.95% or 2.14ppm (1 mg L-1) manganese. The sample is then diluted 3 times giving 1.07ppm, 0.535ppm, and 0.268ppm. Each sample's absorbance was then read using a spectrophotometer were 0.951, 0.491, 0.258, 0.136, respectively. Determine the amount of manganese (in parts per million) of an unknown sample if the absorbance was read to be 0.510.
firstly a callibration curve has to be made using absorption values of samples of known concentration.Then the concentration of unknown sample can be determined from the callibration curve-
data
concentration(ppm) Absorbance
2.14 0.951
1.07 0.491
0.535 0.258
0.268 0.136
unknown 0.510
ans=0.717ppm