In: Chemistry
This is a neutral Amini acid as it contain one free -NH2 and one free - COOH group, to calculate the exact PI value we should know the value of pka of free -NH2of thr and free - COOH of ala. As these are not given so we can take a generalization. Since it is a neutral amino acid it's PI will lie between 5.5 to 6.3 .PI is the pH at which the net charge of a amino acid is zero.