Question

In: Chemistry

You have the following stock solutions and solid chemicals in your lab: 0.15 M NaCl 0.50...

You have the following stock solutions and solid chemicals in your lab: 0.15 M NaCl 0.50 M Tris buffer, pH 8.0 25 mg/ml Bovine serum albumin (BSA) MgCl2 (solid, anhydrous) MW = 95.211 g/mole (95% pure) 1.0 M Glucose
How would you make 250 ml of the following solution:
0.1M NaCl, 0.05M Tris, pH 8.0, 0.25 mg/ml BSA, 100 mM MgCl2, 140 mg% glucose.

Solutions

Expert Solution

queen_honey_blossom answered 2 years ago
Next > < Previous

Related Solutions

Which of the following solutions has the lowest boiling point? EXPLAIN. A) 0.15 M NaCl B)...
Which of the following solutions has the lowest boiling point? EXPLAIN. A) 0.15 M NaCl B) 0.10 M MgBr2 C) 0.15 M Ba(NO3)2 D) 0.20 M C2H6O2 E) 0.10 M Fe(NO3)3
I am provided with the following stock solutions: 1M Tris, pH 8, 5 M NaCl, 1M...
I am provided with the following stock solutions: 1M Tris, pH 8, 5 M NaCl, 1M MgCl2, lysozyme, and DNase. Prepare a 25 mL solution of complete lysis buffer using the following recipe: - 50 mM Tris (pH 8) - 150 mM NaCl - 2 mM MgCl2 - 0.5 mg/mL lysozyme - 0.04 uL/mL DNase In order to perform ion-exchange chromatography, I also need to prepare elution buffers. Using the stock solutions listed above, I need to calculate how to...
A 1.5 kg solid cylinder (radius = 0.15 m , length = 0.60 m ) is...
A 1.5 kg solid cylinder (radius = 0.15 m , length = 0.60 m ) is released from rest at the top of a ramp and allowed to roll without slipping. The ramp is 0.80 m high and 5.0 m long. When the cylinder reaches the bottom of the ramp, what is its total kinetic energy? When the cylinder reaches the bottom of the ramp, what is its rotational kinetic energy? When the cylinder reaches the bottom of the ramp,...
Calculate the molality of each of the following aqueous solutions: (a) 2.01 M NaCl (density of...
Calculate the molality of each of the following aqueous solutions: (a) 2.01 M NaCl (density of solution = 1.08 g/mL) m (b) 51.8 percent by mass KBr solution
Which of the following aqueous solutions are good buffer systems? . a. 0.15 M sodium hydroxide...
Which of the following aqueous solutions are good buffer systems? . a. 0.15 M sodium hydroxide + 0.29 M sodium bromide . b. 0.31 M hydrofluoric acid + 0.20 M potassium fluoride . c. 0.27 M nitric acid + 0.24 M potassium nitrate . d. 0.16 M hydrocyanic acid + 0.18 M potassium cyanide . e. 0.31 M ammonium nitrate + 0.30 M ammonia . b.How many moles of sodium hydroxide would have to be added to 250 mL of...
Arrange the 0.15 M solutions of the following compounds in order of increasing acidity (least acidic...
Arrange the 0.15 M solutions of the following compounds in order of increasing acidity (least acidic first). NH4I                B. CH3COONH4          C. CH3COOK           D. KCl                 E. CH3COOH                    F. KF                   G. HBr                          H. CHCl2COOH        I. NH3                  J. CF3COOH       ammonia,    Kb = 1.80
What does it mean when we say that a 0.15 M NaCl solution is isotonic with...
What does it mean when we say that a 0.15 M NaCl solution is isotonic with blood, whereas distilled water is hypotonic?
Calculate the pH of the following solutions. A.) 0.36 M NH4NO3 B)0.50 M Ca(NO3)2 C.)0.080 M...
Calculate the pH of the following solutions. A.) 0.36 M NH4NO3 B)0.50 M Ca(NO3)2 C.)0.080 M C6H5NH2 D.) 0.45 M KNO2
list the following aqueous solutions in order of their expected freezing point: 0.050m CaCl2, 0.15 m...
list the following aqueous solutions in order of their expected freezing point: 0.050m CaCl2, 0.15 m HCl, 0.10 m HCl, 0.050 m CH3COOH, 0.10 m C12H22011
Determine the pH of the following solutions 8.0×10−2 M RbOH and 0.130 M NaCl 9.2×10−2 M...
Determine the pH of the following solutions 8.0×10−2 M RbOH and 0.130 M NaCl 9.2×10−2 M HClO4 and 2.6×10−2 M KOH 0.120 M NaClO and 4.50×10−2 M KI
ADVERTISEMENT
Subjects
ADVERTISEMENT
Latest Questions
ADVERTISEMENT