Question

Based on a micrsocopy course.

Many emerging advanced imaging methods involve mitigation of out of focus light scattering which is problematic with widefield or confocal imaging. Name 3 different Advanced imaging methods that achieve greater resolution by mitigating out of focus light scatter and state briefly (but specifically) how each method does this.

Answer 1

Three different methods with respective to advanced imaging methods which are used to obtain greater resolution by mitigating out of focus light scattering are as follows -

Analysis of Microbiology(pathology) by advanced imaging methods, information encompasses of high resolution of digital imaging as well as related applications,Which is also known as Optical biopsies with higher resolution.Microscopy is consider as important way of diagnostic of pathology as it provides biomedical information which can be used by scientist and further R&D can be done.

THree such methods are

Phase contrast microscopy - Advanced imaging is provided by differences in refractive index which are used to provide contrast in the unstained microscopic specimens which can be visualized. Light passing through transparent specimen with small phase shifts gets converted intp contrast images as well as in amplitude in the images which provides higher resolution used for observing high living cells in natural or unstained environment.
Fluorescence microscopy (wide field) - In fluorescence microscopy , it uses the fluorescences rather transmitted or reflected light for high contrast and higher resolution images.It is use useful for obtaining different and chossing fluorophores as well as the ability to visualiz multiple fluorophores side by side.

Optical coherence tomography(OCT) and MIcroscopy(OCM) - OCT and OCM provide micron scale images which are based in low coherence interferometry for acheiving high resolution imaging which can be used to observe living tissues in natural as well as unstained state with high 3D volume of images to high speed imaing via in vivo.