Tube No. Digestion (Yes or No) Color Change 1 (EW+Pepsin+Water) No Cloudy, sedimentation 2 (EW+Pepsin+HCl) Yes...

Tube No.	Digestion (Yes or No)	Color Change
1 (EW+Pepsin+Water)	No	Cloudy, sedimentation
2 (EW+Pepsin+HCl)	Yes	Clear, little or no sediment
3 (EW+Pepsin+HCl; in cold)	Some digestion	Clear, more sedimentation
4 (EW+Boiled Pepsin+HCl)	No digestion	A lot more sedimentation
5 (EW+Water+HCl)	No digestion	Cloudy, sedimentation
6 (EW+Pepsin+NaOH)	No digestion	No sediment; pinkish clear

EW = Egg White

What do you suppose is the pH of saliva?

2. What do you think will happen to the activity of salivary amylase in the stomach?

What is the effect of temperature on the activity of salivary amylase? How do you think that this happened?

4. What class of carbohydrates is starch?

If we had used cellulose in this experiment, how could the results have been different?

Expert Solution

1. pH of saliva is neary neutral which ,salivary amylase shows optimum activity in pH 6.7-7.2,Thus salivary gland secretes bicarbonates along with enzymes.

2.Enzymes are highly specific for pH and temperature.Salivary amylase works on pH of 6.7-7.2 ,and stomach secretes Hydrochloric acid which makes the environment of stomach highly acidic about pH of 2 -3 .in highly acidic pH salivary amylase get denatured and inactivated.Its activity is stopped.Temperature also affect the activity of enzymes.Salivary amylase show its maximum activity between 89o F to 98 o F.Increasing temperature will affect the activity of enzyme and on high temperature enzyme get denatured , on low temperature enzyme does not get denatured but its activity is diminshed.

3.Carbohydrates having more than 8 monosaccharide units are called polyssacharide.Starch contains the amylose and amylopectin having the numerous units of alpha-D-glucose .Cellulose is also a polysaccharide having units of Beta-D -Glucose, linked through beta glycosidic linkage .Amylase present in saliva which can digest the alpha glycosidic linkage ,Thus Cellulose will not breakdown into simpler units.

Sydney Mullin answered 1 year ago

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