What is effect of eliminating the ribosome binding site for the gene that encodes the leader...

What is effect of eliminating the ribosome binding site for the gene that encodes the leader peptide? Provide your rationale.

Expert Solution

Ribosomes are the main factories for synthesis of protein . In eukaryotes it is 80S and in prokaryotes it is 70S ribosome which help in translation. Ribosomes have 3 sites A, P & E sites. A for binding of amino acyl tRNA , P for binding peptidyl tRNA and E for exit . For initiating the process of translation ribosomes have to nind to mRNA . Now you have eliminated the ribosome binding site for the gene as a result the mRNA & tRNA complex will not be able to bind to the ribosome, as a result the protein synthesis machinery will be hampered and the peptide chain will not form.

There are certain group of antibiotics which works on this princple eg. TETRACYCLINE which inhibits binding of tRNA & ribosomes and finally stopping protein synthesis of the bacteria. ERYTHROMYCIN prevents translocation in by binding to the ribosome.

Hope you get it. Cheers and all the best!!

gladiator answered 2 years ago

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