what is meant by the “General Elution Problem”.

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Expert Solution

Solution :-

Adjusting the retention factor to improve the resolution between one pair of solutes may lead to unacceptably long retention times for other solutes.

One solution to the general elution problem is to make incremental adjustments to the separation conditions over time. Thus, we choose our initial chromatographic conditions to optimize the resolution for early eluting solutes. As the separation progresses, we adjust the chromatographic conditions to favor the separation of later eluting solutes. In gas chromatography this is accomplished by controlling the column’s temperature; for liquid chromatography the same effect is obtained by increasing the solvent’s eluting strength.

queen_honey_blossom answered 2 years ago

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