Our set of coloured calibration standards can now be transferred to 1 cm cells (shown below)...

Our set of coloured calibration standards can now be transferred to 1 cm cells (shown below) to perform UVvis absorption spectrophotometry in a double beam instrument at the λmax value. What reagent do you use for the reference cell in the double beam spectrometer?

Expert Solution

Reagent should be one which follows the following criteria:

1. As mentioned in the question, the caibration standards are colored which means the reference cell should also have a colored solution which is of a concentration higher than the standards' concentration.

2. Another important point while taking the reagent is that the absorbance for the reference solution is set to 1 or equivalent to 100 % or in other words, transmittance set to zero.

3. The reagent taken for the UV-visible spectrometry experiement should be chemically inert, in a way that the chemical form (or formula) does not change during the course of the analysis.

queen_honey_blossom answered 2 years ago

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