Our set of coloured calibration standards can now be transferred to 1 cm cells (shown below)...

Our set of coloured calibration standards can now be transferred to 1 cm cells (shown below) to perform UVvis absorption spectrophotometry in a double beam instrument at the λmax value. What reagent do you use for the reference cell in the double beam spectrometer?

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Expert Solution

Reagent should be one which follows the following criteria:

1. As mentioned in the question, the caibration standards are colored which means the reference cell should also have a colored solution which is of a concentration higher than the standards' concentration.

2. Another important point while taking the reagent is that the absorbance for the reference solution is set to 1 or equivalent to 100 % or in other words, transmittance set to zero.

3. The reagent taken for the UV-visible spectrometry experiement should be chemically inert, in a way that the chemical form (or formula) does not change during the course of the analysis.

queen_honey_blossom answered 1 year ago

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