Question

In: Biology

6) Fill in the blanks AND put the following steps of DNA replication in the correct...

6) Fill in the blanks AND put the following steps of DNA replication in the correct order

ORDER

DNA Replication Steps

RNA primers are removed and gaps are filled by DNA polymerase.

­­­­­­­­­­­______________________ starts adding nucleotides to the 3' end of the primer.

The gap between the two DNA fragments is sealed by __________________, which helps in the formation of phosphodiester bonds.

Elongation of both the lagging and the leading strand continues. The continuously synthesized strand is known as the _______________strand. The ___________ strand is replicated away from the replication fork, in small fragments known as ______________ fragments, each requiring a primer to start the synthesis.

_________________ binds at the region ahead of the replication fork to prevent supercoiling.

An enzyme called _____________ unwinds the DNA by breaking the ____________ bonds between the nitrogenous base pairs. Two Y-shaped replication forks are formed at the origin of replication and these get extended bi-directionally as replication proceeds.

______________________ binding proteins coat the DNA around the replication fork to prevent rewinding of the DNA.

Primase synthesizes ___________________complementary to the DNA strand.

There are specific nucleotide sequences called ____________ of replication where replication begins.

Solutions

Expert Solution

RNA primers are removed and gaps are filled by DNA polymerase.

­­­­­­­­­­­_DNA polymerase_starts adding nucleotides to the 3' end of the primer.

The gap between the two DNA fragments is sealed by _DNA Ligase_, which helps in the formation of phosphodiester bonds.

Elongation of both the lagging and the leading strand continues. The continuously synthesized strand is known as the _Leading _strand. The _Lagging_ strand is replicated away from the replication fork, in small fragments known as __Okazaki__ fragments, each requiring a primer to start the synthesis.

__Topoisomerases__ binds at the region ahead of the replication fork to prevent supercoiling.

An enzyme called _Helicase_ unwinds the DNA by breaking the __Hydrogen__ bonds between the nitrogenous base pairs. Two Y-shaped replication forks are formed at the origin of replication and these get extended bi-directionally as replication proceeds.

_SSBs__ binding proteins coat the DNA around the replication fork to prevent rewinding of the DNA.

Primase synthesizes _RNA primer_complementary to the DNA strand.

There are specific nucleotide sequences called _Origin_ of replication where replication begins.


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