Question

using three enzymatic steps propose a pathway for the conversion of alpha ketoglutartate to 2-oxoadipate. Draw the missing intermediates and indicate the chemistry involved in each reaction. Include any cofactors that might be required.

Answer 1

ANS.

First step :-

The Alpha Keto gluterate is converted into Alpha Keto adipate by the enzyme Homo citrate dehydrogenase enzyme.

Second step :

The alpha ketoadipate is converted to Alpha hydroxyadipate by the enzyme 2 Keto adipate reductase by converting NADH into NAD redox potential.

Third Step :-

This alpha hydroxy adipate undergoes dehydrogenation to form Hexa 2 enedioate by using enzyme Alpha hydroxy adipate dehydratase by removal of H2O.

Fourth Step :-

This Hexa 2 enedioate undergoes Oxidation reaction to form 2 Oxoadipate by using NADH redox potential by enzyme Oxidoreductase leaving NAD+.

1. The first missing intermediate:

COO^-                                                  

              |

             CH₂

              |

OOC - C – OH

              |

             CH₂

            |

             CH₂

              |

             COO^-

2. The second missing intermediate:

             COO^-                                                 

              |

             CH

              ||

OOC - C

              |

             CH₂

            |

             CH₂

              |

             COO^-

3. The third missing intermediate:

             COO^-                                                 

              |

    H - C- OH

              |

OOC - C- H

              |

             CH₂

            |

             CH₂

              |

             COO