For a given fluorophore, is absorption or emission better suited for life-time measurements?

Expert Solution

In case of measurement of fluorescence life time (FLT) there are three stages. In the first satge the excitation in the form of energy is absorbed by the molecule and the molecule is excited (from S0-->S0) in the second stage there are some geometry relaxation , internel conversion (non-radiative) and intersystem crossing (ISC) will occur in this section the molecule will not emmits any radiation. In the third stage the molecule emmits radiation in the form of fluorescence and the energy is of the emission is less than the energy of the excitation. In the second stage the molecule stayed in a excited state for some nano or hundreds of nano second time which is known as the excited state lifetime and as in this process no emission will takes place thus the time between excitation (simply a LASER pulse) and the emission the time is excited state life time which is completely depends on the energy absorbed by the fluorophore. Thus absorption is more important than emission in the case of FLT.

genius_generous answered 2 years ago

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