In: Biology
Let’s start by assigning letters for our alleles for the two traits: *****These are the letters that I assigned*****
Colony color: Red: RR Cream: rr
.Tryptophan Dependence: Growth: TT No Growth: tt
Now, let’s do our first parental cross of the yeast dihybrid, homozygous parents! Fill in the Punnett Square where the grey is the area to list the gametes generated from each parent (P1) and the white boxes are the F1 offspring
***first row and colum are grey*****
---------->grey |
R |
R |
T |
T |
r |
Rr |
Rr |
Tr |
Tr |
r |
Rr |
Rr |
Tr |
Tr |
t |
Rt |
Rt |
Tt |
Tt |
t |
Rt |
Rt |
Tt |
Tt |
Now, let’s do the F1 x F1 dihybrid cross so we can understand which gametes are going into this cross and we can make some predictions about phenotypes!
R |
r |
T |
r |
|
R |
RR |
Rr |
RT |
Rr |
t |
Rt |
rt |
Tt |
tr |
T |
RT |
Tr |
TT |
Tr |
t |
Rt |
rt |
Tt |
rt |
****Please help, are these correct??? If so what would be the phneotypes and genotypes for both parent and f1generation? Explanation would be appreciated as well****
The correct answers are as follows
TTRR (Red, growth) x rrtt (cream, no growth)----P1
TR rt ---------------------------Gametes
TtRr (Red, growth)------------------F1
F1 x F1
TtRr x TtRr
TR | Tr | tR | tr | |
TR | TTRR (red, growth) | TTRr (red, growth) | TtRR (red, growth) | TtRr (red, growth) |
Tr | TTRr (red, growth) | TTrr(cream, growth) | TtRr (red, growth) | Ttrr(cream, growth) |
tR | TtRR (red, growth) | TtRr (red, growth) | ttRR (red, no growth) | ttRr (red, no growth) |
tr | TtRr (red, growth) | Ttrr(cream, growth) | ttRr (red, no growth) | ttrr (cream, no growth) |
F2 phenotypic ratio:
Red, growth : Red, no growth : Cream, growth : Cream, no growth = 9:3:3:1