Question

What are the differences between Holliday model recombination and double-stranded break repair recombination? What experiments can be conducted to determine which is taking place?

Answer 1

Difference between Holliday model recombination and double-stranded break repair recombination.

Holliday model recombination	Double-stranded break repair      recombination
This model is accounted for the formation of heteroduplex and gene conversion during the recombination.	Double-stranded beak repair is usually done by the homologous recombination. This recombination takes place in the dividing cells through mitosis and meiosis.
Holliday recombination form the Holliday junctions. Two Holliday junctions cut on the crossing strands to form the chromosome without crossover.	The homologous recombination occurs during the DNA repair usually result in the non-crossover products.

Experiment to determine the type of recombination taking place.

Rec A and Rad51 proteins are majorly involved in the general recombination. the homologs of these proteins enable the single strand of the DNA to pair with the homologous region of the DNA double helix.

Targeting these proteins can help in determining the type of recombination taking place.