Question

Based on laboratory testing, it was discovered that the Doubling Time for E.coli at 25^oC is 40 min. Also, based on laboratory testing, it was discovered that E.coli die at 45^oC.

Fill in the table with the estimated number of bacteria:

Time (min)	0	30	60	120	200	400	600
# of E.coli at 25 oC	1
# of E.coli at 37 oC	1

Answer 1

Time	0	30	60	120	200	400	800
#E.coli at 25° C	1	1	2	8	32	1024	1048576
#E.coli at 37°C	1	1	2	8	32	1024	1048576

Temperature do not affect doubling time between an optimum range. Change in pressure increases the doubling time. So same number of E.coli will be found in 25°Cand 37°C after same time intervals. At 37°C growth rate of E.coli is high.