Question

The timing of germination is very important for the survival of a plant species.

If you were trying to discover what the specific mutation in the DNA was that lead to a non-functional GA/aleurone system, which tissues would you use for DNA sequencing analysis? Why?

Answer 1

First you need to know about what GA is ? So

Gibberellins (GAs) are endogenous plant growth regulators, having tetracyclic, diterpenoid compounds

the presence of GA in active growing tissues i.e., shoot apices, young leaves and flowers

So Maily for this testing the part for dna sequencing analysis should be either the seed or embryo part of it

Main reason for it is seed contains embryo that is arrested to develop in to plant with appropriate environmental conditions to continue their life cycles.Breaking of seed dormancy to germination is controlled by some physical factors (light, temperature and moisture) and by the endogenous growth regulating hormones (GA and ABA). GA stimulates the seed germination whereas, ABA is involved in the establishment and maintenance of dormancy. GA exerts its influence in two manners, first by increasing the growth potential of embryo and second by inducing hydrolytic enzymes.During seed germination embryonic GA is released that triggers the weakness of seed cover by stimulating gene expression involved in cell expansion