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In: Biology

Identify each statement in all questions of this section as T (for true statements) or F...

Identify each statement in all questions of this section as T (for true statements) or F (for false statements). Each statement is worth one mark.

  1. Eukaryotic mRNAs contain a poly(A) tail at the 3'-end, but the template DNA encoding the mRNA does not have poly(T)s. Identify the statements as true or false.
  1. The poly A tail is formed by RNA polymerase
  2. The poly A tail is formed by DNA polymerase II
  3. The poly A tail is formed by poly A polymerase
  4. The polyA tail is formed by reverse transcriptase
  5. The enzyme that makes poly A tail is a template-independent polymerase

Solutions

Expert Solution

Answer A. True, eukaryotic mRNAs have a series of adenosines at their 3' end called as poly-A tail which is not specified by the DNA and is added to the transcript by a template-independent RNA polymerase called poly (A) polymerase and this process of addition of adenosine takes place after endonucleolytic cleavage of nascent RNA near the 3'terminus and this process is known as cleavage and polyadenylation or simply called as polyadenylation.

Answer A. The poly-A tail is formed by RNA polymerase- True, the poly-A tail is specified by template-independent RNA polymerase called poly (A) polymerase.

Answer B. The poly-A tail is formed by DNA polymerase II, False. The poly-A tail is specified by template-independent RNA polymerase called poly (A) polymerase. The DNA polymerase II is a monomeric protein and has a 5'-3' polymerase and 3'-5' exonuclease activity and has a role in DNA repair polymerase.

Answer C. The poly-A tail is formed by the poly-A polymerase, True the poly-A tail is specified by template-independent RNA polymerase called poly (A) polymerase.

Answer D. The polyA tail is formed by reverse transcriptase, False, the poly-A polymerase is template-independent but is not a reverse transcriptase. The function of the reverse transcriptase enzyme is to synthesize DNA using an RNA template. The function of reverse transcription is served by telomerase that helps in the synthesis of a DNA strand using an RNA template.

Answer E. The enzyme that makes poly-A tail is a template-independent polymerase, True, poly (A) polymerase doesn't require any template and the substrate for this process is ATP.

Mechanism of polyadenylation is given in figure:

gladiator answered 2 years ago
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