Question

“the phase contrast technique employs an optical mechanism to translate minute variations in phase into corresponding changes in amplitude, which can be visualized as differences in image contrast.” Explain this statement in terms of HOW phase contrast works with its 2 optic rings?

In phase contrast microscopy, why do you have to change the Phase Contrast condenser annulus to MATCH the objective you are using?

Answer 1

Please find the attached answer sheets ( 4 pages )