Cytogenetics of pumpkin

Expert Solution

Cytogenetics is defined as the study of structure and function of chromosomes. Cytogenetic analysis is done to detect any abnormality in chromosome. The pumpkin belongs to the family of cucurbitaceae. This is commonly used as a leaf vegetable in many parts of world like India. The seeds has commercial value as it contain over 40% oil. So the cytogenetic study of pumpkin is important to increase the oil content in seeds, and this can be achived by genetic manipulation. The chromosome number of pumpkin is 2n = 22, which is identified by karyotype study. The cytogenetic study of pumpkin hepls in constructing genetic maps, which is very useful for detecting any diseases related to gene and is helpful for rDNA technology. The karyotype of the pumpkin consists of metacentric and submetacentric chromosomes, indicating less structural differences among the chromosomes, which is indicated by a formula 2n = 22 = 5m + 4sm + 1st + 1t, given in one of the study. Apart from this all the chromosomes are studied for getting a detailed cytogenetic report. These chromosomes are mainly studied at metaphase stage where all the chromosomes are at center of the cell. The cytogenetic study are done by many methods like direct DNA analysis, PCR method, then chromosomal microarray analysis (CMA) which is a molecular cytogenetic method for detecting gains or losses in DNA that are not detectable with normal chromosome analysis. Fluorescent in situ hybridization (FISH) is very useful for detecting any mutation in pumpkin chromosomes.

gladiator answered 1 year ago

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