Assume you have isolated S.aureus from your skin. Design an experiment on how would you determine...

Assume you have isolated S.aureus from your skin. Design an experiment on how would you determine whether it is penicillin and erythromycin resistant? (Make sure to include a hypothesis and how you would design the experiment. Also include proper controls and discuss briefly the techniques you would use). I need one page please.

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Expert Solution

Hypothesis: The S.au is resistant to penicillin and erythromycin.

Experiment: Material and methods: In this experiment we will isolate the S.au from the skin in a swab and let it allow to gro in nutrient media for overnight to get the proper colonies of the S.au. Then we will prepare a Muller Hinton agar media (According to CLSI guidelines). This media will be distributed in five separate flasks

Only media
Media + penicillin
Media + erythromycin
Media+ penicillin + erythromycin
another flask with only media for contol

These media will be poured in petriplates and grouped as above five categories.

Then we will make take few microlitres of the overnight grown culture and spread it on petriplates containing agar media in the following categories

Only media
Media + penicillin
Media + erythromycin
Media+ penicillin + erythromycin

One Petriplate with only media will be left withoutculture as negative contro to check sterility of the media.

All the five plates will be incubated at 37 degree celcius in incubator.

Observation: After overnight, the plates will be observed

Colonies were observed on plate having only media, while the negative control without culture has no colonies.
Few colonies of S. au visible in themedia plates added with penicillin
Few colonies of S. au visible in themedia plates added with erythromycin
Very few colonies of S. au visible in themedia plates added with penicuillin and erythromycin

Inference: The staphylococcus aureus isolated from the skin is able to grow on media supplemented with penicillin and erythromycin separately as well as additively.

This shows that the S.au is resistant to both the antibiotics.

gladiator answered 1 year ago

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