Question

Beginning with the binding of TFIID and extending through the addition of the PolyA tail, please outline the entire process of transcription in eukaryotes. Be sure that you address the transcription factors, binding of RNA Polymerase, the modifications made to the mRNA as transcription is occurring, the CTD tail and the phosphorylation patterns and what significance those have, and alternative splicing.

Answer 1

1.CTD domain of Pol II is Phosphorylated  by the TFIIH kinase activity and chnage in confirmation of Initiation complex and elongation starts.TFIIH also have the DNA repair activity or recognised the DNA damage by halt at the side and recruite the nucleotide exclusion DNA repair complex.

Poly A tail is the addition of poly adenine residues at the 3' end of mRNA and prevent the RNA from degradation and more stable.It is required for RNA stability.Alternative RNA splicing is important to produced the mature RNA which contain only protein coding exons instead of intron and this spliced mature RNA produced the fuctional protein after translation.