Question

Treatment of a person diagnosed with COVID-19 is starting. What test would you recommend to someone who wants to understand whether the treatment is effective?

Answer 1

Covid - 19 testing involves the detection of viruses or the antibodies that are produced for the antigen of the Covid- 19 . The antibody testing detects the starting phase of the infection and able to detect only small concentration of the antibodies . The virus antigen is detected by the process that is known as reverse transcriptase polymerase chain reaction. The RNA of the virus is first converted into the DNA by the enzyme called reverse traanscriptase. It is then passed through the series of reactions that is used for the amplification of the DNA. The process of RT -PCR that is the reverse transcriptase polymerase chain reaction takes few hours to detect the SARS COV- 2 the real time PCR is the advanced technique that are used to detect the corona positive patient . Samples are obtained by rubbing the swab and extracting the sample from the Nasopharangeal secretions or through the saliva. The another test is the isothermal amplification assays. It is also used for amplification of the viral genetic material . Another test includes the ELISA test that is also used in the AIOS. ELISA is the enyme linked immuno sorbent assays. It is based on the intraction between the antigen and the antibodies. There are 2 types of ELISA test that is the direct and the indirect ELISA test. Another test is neutralisation technique is to test the antibody sample prevent the antigen of the SARS CO - 2.  

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