1. What are discrepancies between calf intestinal mucosa alkaline phosphatase and human alkaline phosphatase? 2. A...

1. What are discrepancies between calf intestinal mucosa alkaline phosphatase and human alkaline phosphatase?

2. A solution was made up with p-Nitrophenyl phosphate (pNPP), 0.1 M borate buffer at pH 9.5 and and alkaline phosphatase. This solution has good enzyme activity. Why does changing the pH to 9 decrease the apparent activity of alkaline phosphatase?

3. solution was made up with p-Nitrophenyl phosphate (pNPP), 0.1 M borate buffer at pH 9.5 and and alkaline phosphatase. 50 uM of phosphate was added into the solution. Does the addition of phosphate to the assay mix cause competitive or non-competitive inhibition? Is this expected?

Expert Solution

Please find the answer sheet attached :

gladiator answered 1 year ago

Experiments A and B are 2 experiments performed by mixing alkaline phosphatase enzyme with Pnpp substrate....

Experiments A and B are 2 experiments performed by mixing alkaline phosphatase enzyme with Pnpp substrate. The reaction was run for 5 minutes for each tube, then stopped by adding NaOH. A)Changing enzyme concentration Tube Reaction Buffer (mL) 5.4 mM of pNPP substrate (mL) 0.002 mg/ml of AP enzyme (mL) 3M of NaOH (mL) Absorbance Final Enzyme concentration (mM) Velocity (umol/min/mL) 1 3.41 0.04 0.05 0.875 0.284 2 3.36 0.04 0.1 0.875 0.387 3 3.31 0.04 0.15 0.875 0.509 4...

12. What is the activity of phosphatases, how do acid and alkaline phosphatase differ, and what...

12. What is the activity of phosphatases, how do acid and alkaline phosphatase differ, and what are some possible causes of abnormal levels? 13. What is the activity of lactate dehydrogenase, and what might cause plasma levels to be abnormally elevated? 14. Given the abnormally elevated levels of both alkaline phosphatase and lactate dehydrogenase, is there one particular organ that is likely affected? Explain.

Indicate whether each statement is true or false: 1. The intestinal mucosa produces most of the...

Indicate whether each statement is true or false: 1. The intestinal mucosa produces most of the secretions that enter the small intestine, but the secretions of the spleen and the pancreas also enter the small intestine and play essential roles in digestion. True False 2. The mucus produced by the intestinal and duodenal glands and the goblet cells protects the wall of the intestine from the effects of the acidic chyme and from digestive enzymes. True False 3. Small molecules, which are breakdown products...

1. This procedure recommends preparing E coli as spheroplasts to expose the alkaline phosphatase. Why is...

1. This procedure recommends preparing E coli as spheroplasts to expose the alkaline phosphatase. Why is this a preferred strategy than simply completely lysing the bacteria completely? 2. Explain the unit 'Unit' when attempting to express a quantity of enzyme. Relate your definiton to the reaction taking place in the PNPP reaction.

What could be some of the reasons for the discrepancies between the Hilpert’s equation or the...

What could be some of the reasons for the discrepancies between the Hilpert’s equation or the Churchill & Bernstein’s equation.

1. What are the main differences between the subject areas in human biology, human evolution, and...

1. What are the main differences between the subject areas in human biology, human evolution, and human ecology? What are the Key similarities? 2. From studying nonindustrial societies, what can we learn about enviormental problems like pollution and global warming? 3. What are the main features of a hunter-gatherer lifestyle? What insight does the study of ! Kung hunter-gatherers offer for understanding human biology?

what are the differences and similarities between dry cell, alkaline batteries, and ni-cad batteries

Explain the difference between human brains and 1.) Dolphins 2.) rats

What discrepancies would likely be observed in, maximal voluntary contraction, isometric fatigue, and recovery rates between...

What discrepancies would likely be observed in, maximal voluntary contraction, isometric fatigue, and recovery rates between a Power lifter and a Marathon runner? Why?

1. What is a glycoproteins? 2. Where are they found in human cells?

1. What is a glycoproteins? 2. Where are they found in human cells? 3. List two important functions. 4. How are glycoproteins manufactured in a cell?

Question