Question

From the bloodstream, siRNA will undergo initial distribution through a circulatory system in a few minutes. siRNA undergoes certain pathways to get eliminated from blood. It will get accumulated in the liver and through renal filtration and finally it will be excluded through urine. Many delivery systems blocks the passage of siRNA through kidneys by enfolding in to larger particles of size 20 nm17. Glomerular basement membrane (GBM) is the main component of renal filtration barrier. GBM causes disassembly of catatonic cyclodextrin containing polymer based siRNA nanoparticles, thereby facilitating rapid elimination of siRNA from circulation. siRNA generally assembled by electrostatic interactions between catatonic delivery components but that will be disturbed by GBM ( when siRNA binds with negativity charged GBM) since siRNA normally accumulate in GBM.

??????????????????????

Answer 1

It is not clear what you want to ask. I'm assuming you have issue with understanding the last line of the paragraph i.e

"GBM causes disassembly of catatonic cyclodextrin containing polymer based siRNA nanoparticles, thereby facilitating rapid elimination of siRNA from circulation. siRNA generally assembled by electrostatic interactions between catatonic delivery components but that will be disturbed by GBM ( when siRNA binds with negativity charged GBM) since siRNA normally accumulate in GBM".

Now the explanation- the glomerular basement membrane (GBM) can disassemble cationic cyclodextrin-containing polymer (CDP)-based siRNA nanoparticles and facilitate their rapid elimination from the circulation. It has been shown in reseach studies that siRNA nanoparticles, but not free siRNA, accumulate and disassemble in the GBM. It has also been shown that siRNA nanoparticles do not disassemble in blood plasma in vitro and in vivo, and therefore, this clearance mechanism may affect any nanoparticles that assemble primarily by electrostatic interactions between cationic delivery components and anionic nucleic acids.

I'm not sure if this is really what you needed to be explained, please be more specific next time.

Thanks!