Why is it more advantageous to use Edman degradation instead of Sanger’s sequencing method?

Expert Solution

before discuusing advantages of edman degradation, let us have a look at the disadvantages of Sanger sequencing which are being taken care of by the edman process

Limitations of Sanger Sequencing

It can only be used to sequence short pieces of DNA, about 350 to 1000 base pairs
Quality of a Sanger sequence is not good in the initial bases because of primer binding
Also sequence quality degrades after 700 to 800 bases
If the DNA fragment was cloned before sequencing , the cloning vector sequence may be present in the final sequence.
Database is mandatorily required for identification of the protein sequence

Advantages of using Edman degradation

No sample pre-treatment required .
Identification possible for unknown proteins not registered in databases
Highly efficient to find the protein sequence
Can be used to sequence long pieces of DNA beyond 1000bp
It can differentiate even isobaric amino acids like Leu and Ile.

gladiator answered 3 years ago

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