Question

In: Biology

I am doing a project on the Glycolytic pathway btw Humans & E.coli? I am stuck on my research to find what all enzymes are involved in the glycolysis of E.coli?

I am doing a project on the Glycolytic pathway btw Humans & E.coli? I am stuck on my research to find what all enzymes are involved in the glycolysis of E.coli? For eg humans have these; Hexokinase Glucose-6-Phosphate Isomerase 6-Phosphofructokinase Fructose-bisphosphate aldolase Triose-Phosphate isomerase Glyceraldehyde-3-phosphate dehydrogenase Phosphoglycerate kinase Bisphosphoglycerate mutase Enolase Pyruvate kinase .

So in the same way what does E.coli have?

Solutions

Expert Solution

Glycolysis breaks down glucose to release ATP and NADPH that are essential building blocks of a cell. E.coli has 3 glycolytic pathways i.e., EDP (Entner Doudoroff pathway) which has fewer enzymatic steps and is more thermodynamically favorable than EMPP (Embden Meyerhof Parnas pathway) or OPPP (Oxidative pentose phosphate pathway) but, E. coli does not use EDP for glycolysis.

OPPP requires 7 enzymes that are

  • glucose-6-phosphate dehydrogenase
  • 6-phosphogluconolactonase
  • 6-phosphogluconate dehydrogenase
  • ribose-5-phosphate isomerase
  • ribulose-5-phosphate 3-epimerase
  • transketolase
  • transaldolase

EMPP requires the following enzymes

  • D-glucose-6-phosphotransferase
  • phosphoglucoisomerase
  • phosphofructokinase
  • fructose-1,6-bisphosphatase
  • fructose-1,6-bisphosphate aldolase
  • triosephosphate isomerase
  • glyceraldehyde-3-phosphate dehydrogenase
  • phosphoglycerate kinase
  • phosphoglycerate mutase
  • enolase (phosphopyruvate dehydratase)
  • pyruvate kinase

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