I am doing a project on the Glycolytic pathway btw Humans & E.coli? I am stuck on my research to find what all enzymes are involved in the glycolysis of E.coli?

I am doing a project on the Glycolytic pathway btw Humans & E.coli? I am stuck on my research to find what all enzymes are involved in the glycolysis of E.coli? For eg humans have these; Hexokinase Glucose-6-Phosphate Isomerase 6-Phosphofructokinase Fructose-bisphosphate aldolase Triose-Phosphate isomerase Glyceraldehyde-3-phosphate dehydrogenase Phosphoglycerate kinase Bisphosphoglycerate mutase Enolase Pyruvate kinase .

So in the same way what does E.coli have?

Solutions

Expert Solution

Glycolysis breaks down glucose to release ATP and NADPH that are essential building blocks of a cell. E.coli has 3 glycolytic pathways i.e., EDP (Entner Doudoroff pathway) which has fewer enzymatic steps and is more thermodynamically favorable than EMPP (Embden Meyerhof Parnas pathway) or OPPP (Oxidative pentose phosphate pathway) but, E. coli does not use EDP for glycolysis.

OPPP requires 7 enzymes that are

glucose-6-phosphate dehydrogenase
6-phosphogluconolactonase
6-phosphogluconate dehydrogenase
ribose-5-phosphate isomerase
ribulose-5-phosphate 3-epimerase
transketolase
transaldolase

EMPP requires the following enzymes

D-glucose-6-phosphotransferase
phosphoglucoisomerase
phosphofructokinase
fructose-1,6-bisphosphatase
fructose-1,6-bisphosphate aldolase
triosephosphate isomerase
glyceraldehyde-3-phosphate dehydrogenase
phosphoglycerate kinase
phosphoglycerate mutase
enolase (phosphopyruvate dehydratase)
pyruvate kinase

gladiator answered 1 year ago

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