Question

In: Chemistry

The isoelectric point, pI, of the protein chymotrypsinogen A is 9.1 , while that of ribonuclease...

The isoelectric point, pI, of the protein chymotrypsinogen A is 9.1 , while that of ribonuclease A is 9.45 .

What is the net charge of chymotrypsinogen A at pH 5.1 ?
What is the net charge of ribonuclease A at pH 11 ?

The isoelectric point of proline is 6.3 ; threonine , 5.6 .

During paper electrophoresis at pH 5.5 , toward which electrode does proline migrate? .
During paper electrophoresis at pH 4.5 , toward which electrode does threonine migrate?

Solutions

Expert Solution

queen_honey_blossom answered 2 years ago
Next > < Previous

Related Solutions

The isoelectric point, pI, of the protein elastase is 8.5 , while that of lipase A...
The isoelectric point, pI, of the protein elastase is 8.5 , while that of lipase A is 4.9 . What is the net charge of elastase at pH 5.1 ? What is the net charge of lipase A at pH 2.5 ? The isoelectric point of valine is 5.96 ; leucine , 5.98 . During paper electrophoresis at pH 6.5 , toward which electrode does valine migrate? . During paper electrophoresis at pH 5.5 , toward which electrode does leucine...
The isoelectric point (pI) of a peptide is the pH at which the peptide does not...
The isoelectric point (pI) of a peptide is the pH at which the peptide does not migrate in an electric field. Since the peptide is zwitterionic, there are the same number of positive charges as negative charges on the peptide population. The pI can be estimated fairly accurately (within 0.1 or 0.2 pH units) from the pK values of all the proton dissociable groups in the peptide. Using pK values from the table at the right, estimate the pI value...
1. which of the following tripeptides would be closest to its pI isoelectric point in a...
1. which of the following tripeptides would be closest to its pI isoelectric point in a solution at pH=5 A)HisLysArg B)HisGluAla C)TyrAspGluHis D)GluAspGly 2. Describe the coordination of the Fe2+ ( name the coordination ligands) in both the deoxy and oxymyoglobin states.
1) Estimate the isoelectric point (pI) for the dipeptide Ser-Ile. Round the answer to one decimal...
1) Estimate the isoelectric point (pI) for the dipeptide Ser-Ile. Round the answer to one decimal place. 2) Estimate the isoelectric point (pI) for the tripeptide Gly-Lys-Val. Round the answer to one decimal place. 3)A mixture of Cys, Glu, Tyr, Lys and Ser was applied to a cation exchange column at pH 6. Which of these amino acids eluted from the column first?    Which of these amino acids eluted from the column last?    Tyr Glu Lys Ser Cys
What is the isoelectric point (pI) of the polypeptide given the following pKa values: alpha-COOH pka=...
What is the isoelectric point (pI) of the polypeptide given the following pKa values: alpha-COOH pka= 9.67 alpha-NH3+ pKa= 2.17 guanidium R group pKa= 12.48 carboxylic acid R group pKa= 4.25
Protein Molecular Weight Isoelectric point Does the protein contain heme moiety? 1 68,000 5.5 No 2...
Protein Molecular Weight Isoelectric point Does the protein contain heme moiety? 1 68,000 5.5 No 2 15,000 5.0 No 3 17,500 9.5 Yes Could gel filtration chromatography be used to separate a mixture containing proteins 1 & 3? If gel filtration chromatography can be used, which protein would elute las? After collection fractions from the column, the absorbance of each fraction will be measured in a spectrophotometer - can both proteins 1 & 3 be monitored at 280 nm and...
Why is protein most likely to precipitate at its isoelectric point? Draw pictures to support your...
Why is protein most likely to precipitate at its isoelectric point? Draw pictures to support your explanation.
Examine the tripeptide below: Lys-Glu-Asp Calculate the isoelectric point (pI) of this tripeptide. You can assume...
Examine the tripeptide below: Lys-Glu-Asp Calculate the isoelectric point (pI) of this tripeptide. You can assume that the pKa of the amino terminus is 9.06 and the pKa of the carboxy-terminus is 1.99. a. 2.95 b. 3.99 c. 5.53 d. 6.48 e. 7.31
Protein X, MW 15 kDa, pI 4.5 Protein Y, MW 25 kDa, pI 8.5 Protein Z,...
Protein X, MW 15 kDa, pI 4.5 Protein Y, MW 25 kDa, pI 8.5 Protein Z, MW 75 kDa, pI 5.0 In what order would proteins X, Y, and Z elute from a size exclusion column? Would you expect all three proteins to resolve completely by size exclusion? Briefly, why or why not?
Protein X, MW 15 kDa, pI 4.5 Protein Y, MW 25 kDa, pI 8.5 Protein Z,...
Protein X, MW 15 kDa, pI 4.5 Protein Y, MW 25 kDa, pI 8.5 Protein Z, MW 75 kDa, pI 5.0 In what order would proteins X, Y, and Z elute from a size exclusion column? Would you expect all three proteins to resolve completely by size exclusion? Briefly, why or why not?
ADVERTISEMENT
Subjects
ADVERTISEMENT
Latest Questions
ADVERTISEMENT