What are the primary differences between a laser scanning
confocal and a spinning disk confocal microscope? Consider
(a) the physical design of the microscope
(b) the method of forming an image
(c) the detector that is used to capture the image.
(a) How does the electron microscopy work? Explain briefly
(b) What are the differences between Scanning Electron
Microscopy (SEM) and Transmission Electron Microscopy (TEM)?
What is the difference between A. Scanning Electron Microscope
(SEM) B. Transmission Electron Microscope (TEM) C. Atomic Force
Microscope (AFM). The difference must include the
Principle, Operation, and recent
Applications
Compare and contrast the following: (4 marks each, total 20
marks) Scanning electron microscope and confocal microscope Gated
transport channels and carrier proteins – in membranes Tight
junctions and desmosomes Substrate level phosphorylation and
oxidative phosphorylation Non-cyclic and cyclic
photophosphorylation Cell membranes are selective. Explain how the
lipid bilayer determines permeability and selectivity of the
membrane. Describe how microtubules help vesicles move.
Differentiate the fate of proteins made at the rough
endoplasmic reticulum compared to proteins made in the cystol....
Compare optical microscopy and standard scanning electron
microscopy.
-Describe 4 main advantages SEM can have over optical microscopy
when applied to structural engineering materials, and also mention
2 advantages that optical microscopy may have.
- You want to investigate the fracture surface of a metallic
alloy for structural applications. discuss the relative advantages
and drawbacks of using SEM in BEI mode, SEM in SE mode and optical
microscopy.
)Tabulate how transmission electron microscopy (TEM) differs
from traditional light microscopy regarding (a) sample preparation
(4), (b) processing (4), (c) instrumentation (4), (d) detection (4)
and (e) the structures that can be observed (3), (f) staining (3),
(g) IHC (4) and (h) ISH (4).
In scanning probe microscopy, what is the most common
technology to facilitate
precise scanning? (Describe/draw the operating principle as
well as the technological
application (actual device)).
In scanning probe microscopy, what is the most common technology
to facilitate precise scanning? (Describe/draw the operating
principle as well as the technological application (actual
device))
Microscope Question: What type of microscopy would you use for
each situation situation and why and how does it work
Routine examination of cheek cells in a classroom setting
Tracking the localization of a transgenic protein from DNA
transformed into the cell.
Examining and generating 3D images of very small
ultrastructures of cells
Examining a specimen that is visible to an ordinary light
microscope but in a specimen that is transparent without
staining
You wish to internally observe a complex...