Your neurobiology lab primarily studies gene expression of distinct brain regions to better understand mechanisms behind...

Your neurobiology lab primarily studies gene expression of distinct brain regions to better understand mechanisms behind memory loss. Your task as a new graduate student is to create libraries of Olfactory Bulb and Hippocampus rat brain regions. Propose an experiment (simple steps, flow chart...) to accomplish this.

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The following are the steps are to be followed for the dissection of rat brain

Sacrifice the rat as per the ethical rules. and remove the rat brain .
Wash the rat brain with cold water and add DEPC .
DEPC is used to remove any blood stains on the brain.
On a metal plate , cut the brain into half . Separate the brain into left and right hemispheres.
Remove the olfactory lobe and place it in the liquid nitrogen .
Store the specimen at -80 degree celcius.
Hippocampus is present in the mid brain. Now for collecting the hippocampus , the ventral side of the brain is placed on the top .
The hippocampus is removed from the mid brain and place store at -80 degrees in liquid nitrogen .
In the case of any neuro degenerative disease, the ER protein synthesises defective protein.
To measure the amount of ER protein immunoenzymatic assay is followed.
The tissue obtained is treated with high levels with oestrogen .
If the protein is normal , then there will be more amounts of oestrogen binding sites. If it is abnormal the binding sites will be less .
A monoclonal antibiody is allowed to bind to the protein that contains oestrogen and measured for the amount of protein present in the tissue.
To construct DNA libraries , the tissue is treated with poly A RNA.
Plus/minus screening of cDNA libraries are followed.
Inorder to identify a specific expression in case of normal and abnormal tissue , probes or p[rimers that are complementary are used.
Antibody complementary to that particular probe is used to isolate the DNA or protein.
For isolating DNAS , this method is called nucleic acid hybridisation .
Thus, proteins or DNAs can be isolated specifically in Rat brain.

gladiator answered 1 year ago

Your neurobiology lab primarily studies gene expression of distinct brain regions to better understand mechanisms behind...

Your neurobiology lab primarily studies gene expression of distinct brain regions to better understand mechanisms behind memory loss. Your task as a new graduate student is to create libraries of Olfactory Bulb and Hippocampus rat brain regions. How would gene expression differences in anatomically distinct brain regions in “control” rats help you identify candidate genes possibly involved in loss of memory phenotype?

Your lab is studying gene expression of the tryptophan operon in the bacterium E. coli. In...

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Your neurobiology lab primarily studies gene expression of distinct brain regions to better understand mechanisms behind...

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Your neurobiology lab primarily studies gene expression of distinct brain regions to better understand mechanisms behind...

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