We are able to purify proteins because they differ from each other in various physical or...

We are able to purify proteins because they differ from each other in various physical or chemical properties. List 3 physicochemical properties of proteins that can be used as basis for their separation. Give a method of separation based on each of these properties and say which property the method is based on.

Expert Solution

The physicochemical properties of proteins that can be used as basis for their separation can be
Molecular weightor molecular size, solubility, volatility, ionzation (based on charge), boiling point,
hydrophobicity, specific interaction between enzyme and substrate etc.

Methods of separation based on each of these properties :
1. Gel filtration separates proteins on the basis of their respective molecular sizes
It separates larger proteins from smaller ones because only larger ones can travel fast enough
through the polymer in the chromatography column.

2. Ion-exchange chromatography , here separation of proteins is based on charge.
You either have cationic or anionic column which attract the negative or positive ions
respectively.

3. Reverse HPLC : Proteins are separated based on their hydrophobicities which in turn accounts
for their solubility nature using some organic as well as polar aqueous solvents.

4. Affinity chromatography This method separates mixtures based on specific interaction between
enzymes and substrates or receptor and ligand.

queen_honey_blossom answered 2 years ago

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