Consider a glass electrode or [pH electrode (combination
electrode). a. Identify the reference part. b. Identify the
indicator part. c. Explain how the potential of the electrode is
determined. d. Describe a two-point calibration of the electrode.
e. State any five of the eight possible errors that might occur in
pH measurements with the electrode.
Fill in Table 1 with the resting membrane potentials reported in
different cell types, and different organisms (ie squid, frog, etc)
– Discuss the findings – is the resting membrane potential
different in different tissue types? Is the resting membrane
potential different in the same tissue but from different animals?
Discuss why there are differences and what the likely reason for it
is physiologically.
Table 1: RMPs of different cells
Type of cell
RMP
Source (reference)
Human neuron
-70 mV...
Draw a circulating IgG antibody and a TCR on the membrane of a T
cell. Identify specifically the constant and variable Ig domains on
each molecule, identify each of the protein chains, illustrate
specifically where the antigen interaction takes place and for the
circulating IgG, identify and name the region which is responsible
for the antibody effector functions.
The plasma membrane is a structure common to all cell types and
important in providing the cell with selective permeability.
Identify one way that certain organisms can change the construction
of their plasma membrane given changing environmental conditions
(beside biochemistry)
Identify the component or components that fit the following
criteria from the following list (Indicate all correct
answers)
A. Leukoreduced red blood cells B. Glycerolized red blood
cells C. Irradiated red blood cells
D. Fresh Frozen Plasma
E. Liquid Plasma F. Platelets
G. Cryoprecipitate
1. Stored at 4C
2. Stored at -20C
3. Stored at 37C
4. Stored at 22C
5. Used to raise hematocrit/hemoglobin levels
6. Used to restore multiple clotting factors
7. Used to treat thrombocytopenia
8. Inactivates...
Describe the structure of the cell membrane. Identify 3
different types of components (proteins, lipids, or carbohydrates)
of your choice that are found within the membrane. What is the
function of the components and why is it located within the
membrane?
How
do you calculate the Theoretical standards of the cell potentials
for all 6 (1.0 M) cells listed below?
Cu+2 and Zn
Cu+2 and Pb
Cu+2 and Ni
Zn and Pb
Zn and Ni
Pb and Ni
All of the following is true regarding the cell (plasma)
membrane Except:
a) It has amphipathic transmembrane protein
b) It contains glycolipids and glycoproteins
c) it has large pores to accommodate RNA movement in and out of
the cell
d) the proteins can float in the membrane
e) cholesterol is in the membrane to increase the membrane’s
fluidity
2) If you want to form protein from individual amino acids, you
would
a) dephosphorylate the amino acids
b) use a negative...