Question

Describe why co-transcriptional splicing provides higher fidelity splicing than post-transcriptional splicing.

Answer 1

m-RNA splicing is an essential and complex process for removal of intron sequences and is a key generator for mRNA diversity. Co-transcriptional or post-transcriptional splicing is a set of biological process in eukaryotes where primary m-RNA transcript is modified following transcription of a gene to produce mature m-RNA. Co-transcriptional spilicing modification of mRNA while the transcription of gene is going on, while post-transcriptional splicing means modification of mRNA after the completition of transcription. Compared to post-transcriptional splicing, co-transcriptional spilicing has important functional and regulatory implications. In co-transcriptional spilicing, RNA polymerase II greatly increases efficiency of spliciosome complex for splicing. Thus cooperativity in the interaction of splicing factors with the nasant pre mRNA (primary mRNA) may contribute to the kinetics of co-transcriptional splicing. Both transcription and splicing influence each other to yield a more fit mRNA. In this way co-transcriptional splicing increases fidelity of splicing than compared to post-transcriptional splicing.