Question

I'm being ask to design an experiment about enzymes. I'm not sure about how to do that.

To test for the presence of monosaccharides and reducing disaccharide sugars in food, the food sample is dissolved in water, and a small amount of Benedict's reagent is added. The solution should progress in the colors of blue (with no glucose present), green, yellow, orange, red, and then brick red when there is a large amount of glucose present.

Design an experiment where you would determine how quickly Lactaid works to break down milk sugar at different temperatures and pH. Be specific in your description and create illustrations.

answer the questions below

a. What are enzymes?

b. How do enzymes work?

c. What is lactase?

d. What is Lactaid?

e. What is lactose intolerance?

2. Write down how you will perform these experiments? There are two experiments. (1) How does temperature influence Lactaid’s ability to break down lactose (2) How does pH influence Lactaid’s ability to break down lactose? Include illustrations and be very specific and elaborate on why you are doing 3. Write down what you think will happen in each experiment.

Thank you.

Answer 1

A). What are enzymes

Ans Enzyme-Enzymes are proteins that act as biological catalysts. Which accelerates rate of chemical reactions.

(B). How do enzymes work?

Enzymes acts on molecules known as substrates and the enzyme converts the substrates into different molecules known as products.

(C)What is lactase?

Ans-Lactase is an enzymes and it is essential for complete digestion of whole milk ,it breaks down lactose - a sugar which gives milk its sweetness.

(D) What is Lactaid

Ans - Lacatid - a natural lactase, a natural enzyme that helps break down lactose .

Experiment

This experiment has shown that lactase is an appropriate aid in the treatment of lactose intolerance.

WHY DO THIS:
Most of us know someone who is lactose intolerant. In fact, every human being possesses lactose intolerance. However, each of our tolerance levels is different based on our genes. This experiment was an interesting way to see what someone with lactose intolerance needs to do in order to break down their milk. This allows one to view the reaction of a lactase enzyme with glucose.
Results continued
Introduction:
Materials
The expectation for the results was "Beaker 2 should result in a positive indication that the lactase was successful in breaking down the lactose in the milk. The glucose should turn brown." However, the glucose did not turn brown and instead turned shades of yellow and orange. The lactase still proved to be successful in breaking down the lactose in the milk. Since, with the lactase enzyme the milk was more white with orange bits, while without it the entire substance turned yellow.

RESULTS
Procedure
In order to better understand how milk is broken down in the body, an experiment was conducted using a lactase enzyme to break down milk. The prodedure was a fairly simple routine of adding a lactase enzyme to milk. With the assistance of the Benedicts reagent to identify the reaction, this experiment can be conducted.
What this means: In each trial, after the milk separated the amount of glucose was measured.
1. 300 ml milk
2. Lactase liquid
3.250 ml beakers (6)
4. Bennedicts Reagent
5. Pipettes
6. 825 ml H2O
7. Hot plate
8. Test tubes (3)
9. 500 mL beaker
In two separate beakers, pour 50 ml of milk. Label the beakers 1 and 2. Add the 250 ml of H2O to beaker 1. Then, add the lactase enzyme to beaker 2. Record observations made of both reactions. Pour contents of beaker 1 and 2 into a test tubes. Add ten drops of the benedicts reagent to both test tubes. In the 500 mL beaker, add 250 mL of H2O. Place this beaker on prepared hot plate. Place the test tube into the beaker. After 5 minutes, record observations. Repeat