Which of the following are phenotypes that would result from Tn5 transposition into luxI in Vibrio...

Which of the following are phenotypes that would result from Tn5 transposition into luxI in Vibrio harveyii?

	This strain will produce light when cells are given conditioned media from a wild type strain of Vibrio harveyii
	Inability of strain to produce light when cells are grown by themselves culture
	Conditioned media produced from this strain will complement light production of a luxI mutant

Expert Solution

In vibrio harveyii density dependant expression regulated by the concentration of extra cellular molecules that is called auto inducers. To get the density dependant response , a recombinant clone has been used that re actives the function of a class of spontaneous dim mutants to encode the particular function.

Tn5 transposition into luxl in vibrio harveyii results in the phenotypes that will produce light when cells are given conditioned media from a wild type of strain. It has been noticed that if transposon insertion in one locus ,(LuxO) was similar in intensity at maximum level and also acts independantly so that in the sequence analysis of it revealed that the expressed pencoded this protein , LuxO is similar in amino acid sequence response and shows equal expression as signal transduction protein. The phenotype clearly indicated that LuxO can not affect the expression of luminesence and gives nil effect of its presence so it suggests that in the wild type strain of vibrio harbeyii will produce light in tis conditioned media because interactions with other componants in the Lux signalling system gives relief of expression in this case of phenotypes.

gladiator answered 2 years ago

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