How could you show that adherent phenotype was influenced by RpoS?

Expert Solution

RpoS encodes for a sigma factor that leads to transcription in bacteria as stress response in E.coli. Different environmental cues can lead to expression of this gene. when cells adhere to surfaces and form biofilms the expression of this gene is observed. A wild type E.coli and a mutant for RpoS gene E.coli strain is taken and checked for biofilm formation. this can be done by crystal violet staining of the cells.

in both the cases the cells are allowed to grow for 72 hours to alloe for biofilm formation by adherence on surfaces. then media is removed and washed again to remove unadhered cells. next the attached cells are checked by staining with crystal violet.

It is observed that the wild type cells show a greater staining for crystal violet indicating adherence while the mutant does not show much of staining. this proves that the RpoS gene expression is required for adherence.

(the successful mutation was checked by catalase reaction. in wild type due to presence of RpoS gene there is lot of bubbling indicating catalase activity of RpoS on administering H2O2, this was not observed in case of the mutants. this shows that the mutant strain chosen was working fine)

gladiator answered 2 years ago

In a testcross, if all the offspring show the dominant phenotype, then the individual with the...

In a testcross, if all the offspring show the dominant phenotype, then the individual with the unknown genotype was (only pick one). heterozygous recessive. homozygous recessive. heterozygous. a clone of the other testcross individual. homozygous dominant.

Think of a recent instance where you were influenced by a pricing tactic. This could be...

Think of a recent instance where you were influenced by a pricing tactic. This could be seeing a "2 for 1" offer, a special coupon discount, a time when something was advertised at a particular price but then not available, and the like Consider how you came to learn about the pricing tactic and your initial reaction. Step 2 post to the discussion board addressing the following: In your post, describe the instance you considered for step 1, analyzing your...

Could you show me how you completed this? A state board of directors is trying to...

Could you show me how you completed this? A state board of directors is trying to determine if there is a difference in pay for teachers at its two universities. The first university has 42 teachers with an average pay of $58,500 and a population standard deviation of $3,200. The second university has 51 teachers with an average pay of $60,200 and a population standard deviation of $11,700. Determine if there is a difference using a 0.05 level of significance....

We are doing this problem in R software. If you could also show how to do...

We are doing this problem in R software. If you could also show how to do it in that along with the normal work that would be much appreciated. Problem 1 Acid precipitation id linked to the disappearance of sport fish and other organisms from lakes. Sources of air pollution, including automobile emissions and burning fossil fuels, add to the natural acidity of precipitation. The Wisconsin Department of Natural Resources initiated a precipitation monitoring program with the goal of developing...

If all we do is forget in a study, how could you show which theory is...

If all we do is forget in a study, how could you show which theory is correct? Would you be able to tell the difference between the types of forgetting theories?

You cross a mutant with phenotype 'a b c^+' to a mutant with the phenotype 'a^+b^+c'...

You cross a mutant with phenotype 'a b c^+' to a mutant with the phenotype 'a^+b^+c' and test cross the F1. Given this genetic map, predict the phenotypes observed in the offspring, and the number of each expected in a population of 2500 (show your work). c < 19 m.u. > a <8 m.u. > b

Decision #2: Planning for Retirement *If you could show how you got the answers using EXCEL...

Decision #2: Planning for Retirement *If you could show how you got the answers using EXCEL that would be great!* Thanks! Todd and Jessalyn are 25, newly married, and ready to embark on the journey of life. They both plan to retire 45 years from today. Because their budget seems tight right now, they had been thinking that they would wait at least 10 years and then start investing $2400 per year to prepare for retirement. Jessalyn just told Todd,...

Could you show me how to convert this problem? A physician has ordered 5600 mg of...

Could you show me how to convert this problem? A physician has ordered 5600 mg of penicillin V, intramuscularly. If penicillin V were available as 0.80 g/mL of solution, how many milliliters would you need to give?

Could you show me how to form PLAs for the following question? Thanks. 7. Draw a...

Could you show me how to form PLAs for the following question? Thanks. 7. Draw a PLA for the following functions, given in S notation, using variables x0, x1, x2, x3. f1 = S (0, 3, 5, 7, 8, 11, 13) f2 = S (1, 2, 3, 6, 11, 13, 14, 15) f3 = S (0, 2, 4, 6, 7, 12, 13, 15) f4 = S (1, 4, 5, 6, 9, 10, 12) f5 = S (2, 3, 4, 5,...

Plot a graph and write a paragraph to show how a Pigovian fee could work. Show...

Plot a graph and write a paragraph to show how a Pigovian fee could work. Show two different MACs and discuss whether equi-marginal principle holds. If yes, why? If no, why not?

Question